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. 2021 Mar 4;12(3):238. doi: 10.1038/s41419-021-03517-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Relative mRNA expression levels of USP53 during the osteogenic differentiation of hBMSCs. HPRT was used for normalization. b Protein levels of USP53 during the osteogenic differentiation of hBMSCs. Heat shock protein 90 (HSP90) was used as a loading control. USP53 protein expression was quantified using ImageJ. Day 0: undifferentiated hBMSCs. Results are means ± SDs. ns: not significant, *P < 0.05, ***P < 0.001 by one-way ANOVA. n = 3.