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. 2021 Mar 4;11:22. doi: 10.1186/s13550-021-00762-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Workflow and tissue sample processing from 6 [^99mTc]Tc-PSMA RGS lymphadenectomies. Resected tissue specimens from subregions were manually separated to the level of “single samples” (aiming at single LN) that were measured with a high-purity germanium detector. After final histopathological analysis, 160 LNM and 11 non-nodal tumor samples were present in 154 manually separated single samples and 516 tumor-free lymph nodes were enumerated out of 252 tumor-free single samples