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. 2021 Mar 4;12:1443. doi: 10.1038/s41467-021-21663-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, d Cartoon illustrating the hnRNP L and SETD2 constructs along with the known domains that were used in affinity-purifications and in vitro binding. b Halo purification was performed from extracts of 293T cells co-expressing Halo-tagged SETD2C and mCherry-HA-hnRNP L. Input and eluted samples were resolved on gel followed by western blotting. The expected band for the target proteins are depicted by arrows. RNase treatment was not performed for these experiments. The experiment was repeated at least two times all yielding similar results. c Microscopy images showing the localization of mCherry-hnRNP L constructs. The scale bar is 10 µm. The experiment was repeated at least four times all yielding similar results. e GST pull-down was performed using recombinant proteins purified from bacteria. RNase was included in the binding assay. The input and eluted samples were resolved on gel followed by western blotting with the depicted antibodies. The experiment was repeated at least two times all yielding similar results. AWS associated with SET, SET Su(var)3–9, Enhancer-of-zeste and Trithorax, SRI Set2-Rpb1 interaction, SHI SETD2-hnRNP interaction, RRM RNA-recognition motif, NLS nuclear localization signal, GST glutathione-S-transferase.