Figure 1.
Identification of distinct mitochondrial morphologies in primary neurons. (A) Representative immunofluorescent image of mitochondria labeled for ATP synthase (green) and TOM20 (red). Insert: mitochondrial objects segmented and color coded by classified morphology. (B) Principal component analysis (PCA) of hand-classified mitochondrial objects in the training and test sets combined. Individual objects are color coded by morphology (n = 1091 mitochondrial objects). (C) Heatmap displaying the scaled 32 size and shape measurements by morphology. (D) Confusion matrix displaying the results of model evaluation using the hand-classified test set (217 objects).