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. 2021 Mar 4;11:5133. doi: 10.1038/s41598-021-84528-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Morphological analysis of mitochondria in Opa1 conditional knockout. (A) Representative images of primary Opa1^flx/flx neurons (top) and Opa1^flx/flx neurons with Cre (bottom). Panels (from left to right): ATP synthase (green) and TOM20 (red) merged, MAP2 (magenta), DAPI (blue), all channels merged. Scale bars = 10 µm. (B) Percentage of mitochondrial area per morphology in Opa1^flx/flx cells (black) and Opa1^flx/flx cells with Cre (gray). Mean ± SD (n = 9 per group). * indicates p < .05. (C) Representative Western blot displaying Opa1 and GAPDH protein levels across Cre-Lentivirus concentrations. Full-length blots are presented in Supplementary Fig. 3. (D) Western blot quantification of Opa1 protein levels after Cre-Lentivirus treatment (n = 3). For all Opa1 cKO experiments, 1 × MOI Cre-Lentivirus was used.