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. 2021 Mar 4;11:5133. doi: 10.1038/s41598-021-84528-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Morphological analysis of mitochondria in glutamate and cyclosporin A (CsA) + Ru360 experiments. (A) Representative images of primary cortical neurons. Rows (top to bottom): vehicle-treated control group, control cells treated with CsA + Ru360 only, vehicle-treated group with 30 min 100 µm glutamate exposure, glutamate challenged group pre-treated and co-incubated with CsA + Ru360 Panels (from left to right): ATP synthase (green) and TOM20 (red) merged, MAP2 (magenta), DAPI (blue), all channels merged. Scale bars = 10 µm. (B) Percentage of mitochondrial area per morphology in glutamate and CsA + Ru360 experiments. Mean ± SD (n = 6–8 per group). * indicates p < .05 versus Control. # indicates p < .05 versus Control + CsA + Ru360. $ indicates p < .05 versus Glutamate.