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. 2021 Mar 4;11:3719. doi: 10.1038/s41598-021-83239-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Two key aspects of a daisyfield gene drive: no daisy, no drive, and the halving of the daisyfield each mating with a wildtype individual. If there are no daisy elements in the genome, there is no drive due to the absence of a gRNA. Daisy elements are scattered throughout the genome (forming a daisyfield) and inherited in a Mendelian fashion. Therefore, offspring inherits half of the daisy elements from each parent. Thus, after enough matings with wildtype individuals, there will be no daisy elements left. (b) Population size over time after the introduction of 100 squirrels with a HD-ClvR gene drive to a population of carrying capacity 3000. Lines represent the average population size over 100 model replications, while opaque ribbons represent the 95% quantiles. The model was run with an NHEJ rate ( $P_{n}$ ) of 0.02, 1 homing gRNA, and 4 cleave-and-rescue gRNAs. Gene drive squirrel supplementation was done yearly, the amount being a percentage (0, 1, or 10%) of the total population size at that moment.