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. 2021 Mar 4;11:3719. doi: 10.1038/s41598-021-83239-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Spatial dynamics of HD-ClvR using different placement schemes. (a) A schematic overview of the placement schemes. (b) Population size as a function of the placement schemes and amounts of supplementation. We modelled population size over time after the introduction of 100 squirrels with a HD-ClvR gene drive with 1 homing gRNA and 4 cleave-and-rescue gRNAs to a population of carrying capacity 3000. We modelled an NHEJ rate ( $P_{n}$ ) of 0.02 and a daisyfield of size 30. (c) Three snapshots of moments representing key spatial dynamics at 10% supplementation. See the full animations in Supplementary Videos S7.