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. 2021 Mar 4;11:5113. doi: 10.1038/s41598-021-84564-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of hESC-derived colon organoids. (A) Immunostaining of H9 hESC-derived definitive endoderm (day 4) and hindgut endoderm (day 8). Scale bars: 200 µM. (B) Representative bright-field images of developing hESC-derived colon organoids. Scale bars: 200 µM. (C) Immunostaining of hESC-derived colon organoids. Adult human colon tissue samples and H9 hESCs served as controls. Scale bars: 200 µM. (D) RT-PCR analysis of colonic cell markers in hESC-derived D28 colonic spheroids and D40 colon organoids. Adult human colon tissue samples and H9 hESCs served as positive and negative controls, respectively. Data are presented as relative mean expression (± standard deviation) of triplicate samples from one experiment.