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. 2021 Mar 4;11:5113. doi: 10.1038/s41598-021-84564-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cell proliferation in FAP-hESCs and their derived colon organoids. (A) Representative photos of immunohistochemical staining of the cell proliferation marker Ki67 (right) and the colon epithelial marker CDX2 (left) in control and FAP-colon organoids on day 48 of differentiation. Scale bars: 100 µM. (B) Quantification of Ki67/CDX2-positive cells was performed using the image processing program ImageJ, using ImmunoRatio software, on 5 different fields taken from each sample (magnification: 10 ×). One-way ANOVA *p < 0.05, **p < 0.01. (C) Representative images of the Ki67 immunostaining pattern in normal control colonic mucosa (WT) versus normal-appearing crypts from FAP patients (APC + / −). Scale bars = 100 µM.