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. 2021 Mar 4;11:5265. doi: 10.1038/s41598-021-83894-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo confocal microscopy allowed detailed analysis of cell structure at each time- point in healthy and pneumonia lungs with cell size differences discernibly evident at 24 h between MSCs in healthy and pneumonia lungs. The formation of micro-particles from the cells was indicated in post mortem confocal microscopy (A) and performing IVM using a 40X objective demonstrated the time-lapse release of fluorescent micro-particles (B, Red Arrow) approximately 40 min post cell administration IV (B i-iii) IVM: Intravital Microscopy; MSC: Mesenchymal Stem Cell.