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Tight junction proteins were reduced following repeated low-level BOP exposure. (A) The tight junction protein claudin-5 was reduced (p = 0.11) in the brain following 14 exposures to low-level BOP. (B) Representative Western blot image of claudin-5 and β-actin. (C) Occludin was significantly reduced in the brain following 14 exposures to 8.5 psi overpressure. (D) Representative Western blot image of occludin and β-actin. ***p < 0.001.
