TABLE 3.

PHA production from recombinant cyanobacteria under different cultivation conditions.

Organism	Recombinant factor/Culture media	Yield % (dry cell weight)	Biopolymer	References
CO2 as substrate: Photoautotrophic cultivation
Synechocystis	Random UV mutagenesis	37	PHB	Kamravamanesh et al., 2018a
	Optimized ribosome binding site for phaB1	35	PHB	Wang et al., 2018
	Overexpression of native phaA and phaB Nitrogen deprivation	26	PHB	Khetkorn et al., 2016
	Overexpression of the sigma factor sigE Nitrogen limitation	25	PHB	Osanai et al., 2013
	agp gene—deletion-mutant	14.9	PHB	Wu et al., 2002
	Overexpression phaCCs, nphT7Ss, and phaBCn under the control of the psbAII promoter Increased CO2	14–16%	PHB	Lau et al., 2014
	xfpk overexpression in double pta and ach mutant	12	PHB	Carpine et al., 2017
	Overexpression of PHA biosynthetic operon from Microcystis aeruginosa	7.0	PHB	Hondo et al., 2015
	Knockout mutant of the sll0783 gene Nitrogen limitation	NA	PHB	Schlebusch and Forchhammer, 2010
Synechococcus	Pha genes in complemented cyanobacterial recA null mutant with the E. coli recA Nitrogen limitation	52	PHB	Akiyama et al., 2011
	PHA biosynthesis genes from C. necator	1.85	PHB	Takahashi et al., 1998
Exogenous substrates: Heterotrophic/mixotrophic cultivation
Synechocystis	Overexpression phaCCs, nphT7Ss and phaBCn under the control of the psbAII promoter Acetate + gas exchange limitation	41	PHB	Lau et al., 2014
	Overexpression of native phaA and phaB Acetate and nitrogen Limitation	35	PHB	Khetkorn et al., 2016
	agp gene—deletion-mutant Acetate	18.6	PHB	Wu et al., 2002
	PHA biosynthetic genes of C. necator Acetate and nitrogen limitation	11	PHB	Sudesh et al., 2002
	PHA biosynthetic operon from C. necator Acetate and nitrogen limitation	11	PHB	Sudesh et al., 2001
Synechococcus	PHA biosynthesis genes from C. necator Acetate and nitrogen limitation	26	PHB	Takahashi et al., 1998