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. 2021 Feb 19;9:639006. doi: 10.3389/fcell.2021.639006

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Copyright © 2021 Cao, Zhan, Ci, Wang, She, Xu, Xiao, Xia, Shen, Meng and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological evaluations of the samples in control and biphasic groups at 12 weeks post-operatively. HE staining images of control (A–A2) and biphasic (F–F2) groups. Toluidine blue staining images of control (B–B2) and biphasic (G–G2) groups. SO/FG staining images of control (C–C2) and biphasic (H–H2) groups. Collagen II immunohistochemical staining images of control (D–D2) and biphasic (I–I2) groups. Collagen I immunohistochemical staining images of control (E–E2) and biphasic (J–J2) groups. The black arrows separate native cartilage and neocartilage. The red arrow indicate neo-bone matrix.