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UPLC profile of an extract of human RPE [donor age 74, 1 eye (A, B)]. The chromatogram represents analysis of six eyes. A: Chromatogram with monitoring at 430 nm absorbance. Top insets: UV-visible absorbance spectra of isoA2E, A2E, A2-GPE, A2-DHP-PE, atRALdi-PE, and lysoA2PE species. B: Fluorescence monitoring at an excitation of 430 nm and emission of 600 nm.
