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. 2021 Feb 19;12:640919. doi: 10.3389/fpls.2021.640919

FIGURE 1.

FIGURE 1

N-glycosylation and N-glycan maturation steps in plants. Several Asn-linked glycosylation (ALG) enzymes catalyze the assembly of the dolichol pyrophosphate (PP)-linked oligosaccharide in the cytosol and ER. The multi-subunit oligosaccharyltransferase (OST) complex transfers the oligosaccharide to accessible Asn-residues of nascent proteins. N-glycan processing enzymes: α-glucosidase I (GCSI), α-glucosidase II (GCSII), ER α-mannosidase I (MNS3), Golgi α-mannosidase I (MNS1/MNS2), β-(1 → 2)-N-acetylglucosaminyltransferase I (GnTI), β-(1 → 2)-N-acetylglucosaminyltransferase II (GnTII), β-(1 → 2)-xylosyltransferase, core α-(1 → 3)-fucosyltransferase (FUT11/FUT12), β-(1 → 3)-galactosyltransferase 1 (GALT1), and α-(1 → 4)-fucosyltransferase (FUT13). Terminal GlcNAc residues can be removed by β-hexosaminidases (HEXO1/3). GHs and GTs are listed in Tables 1, 2 while probes against N-glycans are listed in Table 3.