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Glycan array assay for GT characterization. Glass slides equipped with plant cell wall-related oligosaccharides are incubated with azido-functionalized sugar nucleotides and GT candidates expressed in, for example human embryonic kidney (HEK) 293 cells. Any transferred monosaccharide is visualized by azide-alkyne cycloaddition reaction with a fluorescent dye to determine reactive acceptors (reprinted from Ruprecht et al., 2020).
