TABLE 2.
Selected examples for carbohydrate GHs and lyases acting on N-glycans, O-glycans including type-II AGs on AGPs and EXTs.
| Activity | CAZy family | Protein name | Species | References |
| GHs in N-glycan processing and degradation | ||||
| α-(1 → 2)-glucosidase I | 63 | GCSI/KNF-14 | A. thaliana | Boisson et al., 2001; Gillmor et al., 2002 |
| α-(1 → 3)-glucosidase II | 31 | GCSII/RSW3 | A. thaliana | Burn et al., 2002 |
| ER α-(1 → 2)-mannosidase | 47 | MNS3 | A. thaliana | Liebminger et al., 2009 |
| Golgi α-(1 → 2)-mannosidase I | 47 | MNS1/MNS2/GMI | A. thaliana | Liebminger et al., 2009 |
| Glycine max | Nebenführ et al., 1999 | |||
| Golgi α-(1 → 3/6)-mannosidase II | 38 | GMII/HGL1 | A. thaliana | Strasser et al., 2006 |
| α-(1 → 3/4)-fucosidase | 29 | FUC1 | Prunus dulcis (almond), A. thaliana | Zeleny et al., 2006; Kato et al., 2018 |
| β-(1 → 3/4)-galactosidase | 35 | BGAL1 | Nicotiana benthamiana | Kriechbaum et al., 2020 |
| β-hexosaminidases | 20 | HEXOs | A. thaliana | Strasser et al., 2007a; Liebminger et al., 2011 |
| Lytic enzymes acting on type-II AGs glycans of AGPs | ||||
| FvEn3GAL | 16 | FvEn3GAL | Flammulina velutipes | Kotake et al., 2011 |
| exo-β-(1 → 3)-galactanase | 43 | Il1,3Gal | Irpex lacteus | Tsumuraya et al., 1990; Kotake et al., 2009 |
| 43 | A. thaliana | Nibbering et al., 2020 | ||
| endo-β-(1 → 6)-galactanase | 30 | Tv6GAL | Trichoderma viride | Kotake et al., 2004 |
| 30 | Nc6GAL | Neurospora crassa | Takata et al., 2010 | |
| β-(1 → 3),(1 → 6)-galactanase | 35 | RsBGAL1 | Raphanus sativus | Kotake et al., 2005 |
| β-(1 → 3),(1 → 6)-galactanase | 35 | SlTBG1 | Solanum lycopersicum | Eda et al., 2014 |
| α-L-arabinofuranosidase | 54 | NcAraf1 | Neurospora crassa | Takata et al., 2010 |
| 3 | RsAraf1 | A. thaliana, Raphanus sativus (radish), and Bacteroides thetaiotaomicron | Kotake et al., 2006 | |
| Bacteroides thetaiotaomicron | 127 | Cartmell et al., 2018 | ||
| β-L-arabinopyranosidase | 27 | SaArap27A | Streptomyces avermitilis | Ichinose et al., 2009 |
| 27 | AtAPSE | A. thaliana | Imaizumi et al., 2017 | |
| β-glucuronidase | 79 | NcGlcAase | Neurospora crassa | Konishi et al., 2008 |
| 79 | AnGlcAase | Aspergillus niger | ||
| 79 | AtGUS2 | A. thaliana | Eudes et al., 2008 | |
| 4-5-anhydro-glucuronidase | 154 | Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron | Cartmell et al., 2018 | |
| 105 | Cartmell et al., 2018 | |||
| α-L-rhamnosidase | 28 | SaRha78A | Aspergillus niger | Martens-Uzunova et al., 2006 |
| 78 | Streptomyces avermitilis | Ichinose et al., 2013 | ||
| 106 | Sphingomonas paucimobilis | Miyata et al., 2005) | ||
| α-L-rhamno-glucurono lyase | PL27 | Bacteroides cellulosilyticus | Cartmell et al., 2018 | |
| exo-α-L-(1 → 2)-fucosidase | 95 | AfcA | Bifidobacterium bifidum | Katayama et al., 2004 |
| 95 | Aspergillus nidulans | Pogorelko et al., 2016 | ||
| ? | Xanthomonas manihotis | Wong-Madden and Landry, 1995 | ||
| Lytic enzymes acting on glycans in EXTs | ||||
| β-L-arabinofuranosidase | Bifidobacterium bifidum | Fujita et al., 2014 | ||
| β-L-(1 → 2)-arabinofuranosidase | 121 | HypBA2 | Bifidobacterium bifidum | Fujita et al., 2011 |
| β-L-(1 → 2)-arabinofuranosidase | 121 | XeHypBA2 | Xanthomonas euvesicatoria | Nakamura et al., 2018 |
| (XCV2729) | ||||
| α-L-(1 → 3)-arabinofuranosidase | 43 | XeHypAA (XCV2728) | Xanthomonas euvesicatoria | Nakamura et al., 2018 |
| Arabinofuranosidase-(1 → 4)-Hyp | 127 | XeHypBA1 (XCV2724) | Xanthomonas euvesicatoria | Nakamura et al., 2018 |
Please also see Silva et al. (2020) for GHs acting on type-II AG O-glycans of AGPs.