TABLE 3.

Toolkit Abs/probes available to characterize N- and O-glycans.

mAbs/reagent	Species origin	Tissue origin	Minimal epitope recognized	References
JIM4	Daucus carota	Suspension cultured cells	β-GlcA-(1 → 3)-α-GalA-(1 → 2)-Rha	Knox et al., 1989; Yates et al., 1996
JIM8	Beta vulgaris	Suspension cultured cells	unknown	Pennell et al., 1991
JIM13	Daucus carota	Suspension cultured cells	β-GlcA-(1 → 3)-α-GalA-(1 → 2)-Rha	Knox et al., 1991; Yates et al., 1996
JIM14	Daucus carota	Suspension cultured cells	β-Gal-(1 → 6)-β-Gal-(1 → 6)-β-Gal-(1 → 6)	Knox et al., 1991; Yates et al., 1996; Ruprecht et al., 2017
JIM15	Daucus carota	Suspension cultured cells	unknown	Knox et al., 1991; Yates et al., 1996
JIM16	Daucus carota	Suspension cultured cells	β-Gal-(1 → 6)	Knox et al., 1991; Yates et al., 1996; Ruprecht et al., 2017
			β-Gal-(1 → 3)-β-Gal-(1 → 3)-β-Gal-(1 → 3)
JIM84	Daucus carota	Suspension cultured cells	α-L-Fucp-(1 → 4)-β-D-Galp-(1 → 3)-β-D-GlcpNAc-R	Horsley et al., 1993
JIM101	Gymnocolea inflata	Extracted AGPs	unknown	Pattathil et al., 2010
JIM133	Zinnia elegans	Tracheary element cell walls	β-Gal-(1 → 3)-β-Gal-(1 → 3)-β-Gal-(1 → 3)	Pattathil et al., 2010; Ruprecht et al., 2017
LM2	Oryza sativa	Suspension cultured cells	β-GlcA-(1 → 6)-β-Gal-(1 → 6)-β-Gal-(1 → 6)-β-Gal-(1 → 6)	Smallwood et al., 1996; Ruprecht et al., 2017
LM14	Arabidopsis thaliana	Mixed leaves, stems and roots	Unknown	Møller et al., 2008
MAC204	Pisum sativum	Peribacteroid membrane	Unknown	Bradley et al., 1988
MAC207	Pisum sativum	Peribacteroid membrane	β-GlcA-(1 → 3)-α-GalA-(1 → 2)-Rha	Pennell et al., 1989; Yates et al., 1996
PN16.4B4	Nicotiana glutinosa	Suspension cultured cells	Unknown	Norman et al., 1986; Pattathil et al., 2010
β-Glc Yariv (synthetic dye)			β-Gal-(1 → 3)-β-Gal-(1 → 3)-β-Gal-(1 → 3)-β-Gal-(1 → 3)-β-Gal)n>5	Yariv et al., 1967; Kitazawa et al., 2013; Paulsen et al., 2014