Figure 4.
The representative results of Hematoxylin-eosin stating of lung tissue (A), Verhoeff-van Gieson staining (B), and double immunohistochemical staining of smooth muscle actin (green) and PCNA (brown) (C). (D) The ratio of medial wall thickness of pulmonary arterioles. The value for the sham42 group was set at 1.0. The muscular layer of pulmonary arterioles (50–100 μm in diameter) was significantly thicker in the SuPNx42 rats than in sham42, SuPNx42/DIZE1−42, but not SuPNx42/DIZE29−42 rats (n = 6). (E) the vascular occlusion score (VOS). The VOS of early treatment group (SuPNx42/DIZE1−42) was decreased compared to SuPNx42 (1.27 ± 0.74 vs. 1.07 ± 0.56, n = 6) (magnification, 200x. Values represent the mean ± SEM. *P < 0.05, **P < 0.01.