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. 2021 Feb 19;8:619133. doi: 10.3389/fmed.2021.619133

Figure 5.

Figure 5

(A) A representative Western blot of eNOS, ACE, ACE2, and the MAS receptor in sham42, SuPNx42, SuPNx42/DIZE1−42, and SuPNx42/DIZE29−42 rats. Representative results from one of three separate Western blotting experiments are shown. (B) The normalized eNOS/actin ratio revealed the expression of eNOS was higher in the SuPNx42/DIZE1−42 rats than in SuPNx42 rats. (C) The normalized ratio of ACE/actin showed that the expression of ACE was higher in SuPNx42 rats than in sham42 rats. DIZE administration decreased ACE expression in the early treatment (SuPNx42/DIZE1−42) and the late treatment (SuPNX42/DIZE29−42) groups. (D) The normalized ratio of ACE2/actin revealed that an increase in the early treatment group (SuPNX42/DIZE1−42) compared to the SuPNx42 rats. No significant differences were found among the sham42, SuPNx42, and SuPNX42/DIZE29−42 groups. (E) The normalized MAS/actin ratios were not changed in the all groups. The value for the sham42 group was set at 1.0 in (B–E). Values represent the mean ± SEM *P < 0.05, **P < 0.01 (n = 6–7).