Crystallization Buffer

Reagent (stock)	Final concentration	Amount
Bis-Tris-HCl [pH 6.5], HEPES-NaOH [pH 7.0 or 7.5], or Tris-HCl [pH 8.0 or 8.5] (1 M)	100 mM	1 mL
PEG 3350 (50%)	25%	5 mL
ddH2O	N/A	4 mL
Total		10 mL