Reagent | Final concentration | Amount |
---|---|---|
HEPES-NaOH [pH 7.5] (1 M) | 100 mM | 1 mL |
PEG 3350 (50%) | 20% | 4 mL |
ddH2O | N/A | 5 mL |
Total | 10 mL |
Reagent | Final concentration | Amount |
---|---|---|
HEPES-NaOH [pH 7.5] (1 M) | 100 mM | 1 mL |
PEG 3350 (50%) | 20% | 4 mL |
ddH2O | N/A | 5 mL |
Total | 10 mL |