FIGURE 4.

Quantitative Methylation-specific PCR and real-time PCR confirms HSPAIL promoter hypomethylation-overexpression with SARS-CoV-2 infection. Methylation levels of HSPAIL and ULBP2 were measured by qMSP. (A,B) For the HSPA1L gene, the methylation levels were significantly lower but mRNA expression was significantly higher in both asymptomatic and symptomatic COVID-19 compared to healthy controls. (C,D) No difference in ULBP2 methylation levels or mRNA expression was observed between the blood of healthy and COVID-19 samples. *P-value < 0.05 (n = 5 for each group); the red error bar shows standard error of mean (SEM) and the black horizontal bar shows the mean value.