Figure 1.

Aggravation of mechanical allodynia and upregulation of DRG Epac1, NR2B and Piezo2 expression in mice with BCP over time. Intra-femur implantation of osteosarcoma cells induced mechanical allodynia in the ipsilateral hind paw. (A) PWMT was measured at the day before modeling (day-1), and days 4, 7, 10 and 14 in sham and tumor group mice (n=8/group). (B and C) Expression level of Epac1, p-NR2B and Piezo2 of the ipsilateral lumbar DRG L2-L5 segments were detected with western blotting (n=3/group) or reverse transcription-quantitative PCR (n=3/group) at the corresponding time point. Data represents means ± SD. ^#P<0.05 vs. day-1; *P<0.05 vs. sham group at each time point. BCP, bone cancer pain; DRG, dorsal root ganglion; PWMT, paw withdrawal mechanical threshold.