Figure 3.

Exacerbation of mechanical allodynia and upregulation of DRG Piezo2 expression by selective activation of Epac1 or NR2B in normal mice. Normal mice were randomly divided into three groups (n=8/group) and received intrathecal (i.t.) saline (5 µl) and NMDA (1 nmol/5 µl), or intraplantar saline (5 µl) and 8-pCPT (12.5 nmol/5 µl), respectively. (A) PWMT was measured at 0 (before injection), 5, 15, 30, 60 and 90 min after intrathecal or intraplantar injection. (B-D) The expression level of the right lumbar DRG L2-L5 segments of Epac1in 8-pCPT group, p-NR2B in NMDA group and Piezo2 mRNA in both groups were detected at the time of 0, 15 or 30 min after injection. Data represents means ± SD. ^#P<0.05 vs. 0 min; *P<0.05 vs. vehicle group at each time point. DRG, dorsal root ganglion; PWMT, paw withdrawal mechanical threshold.