REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rat anti-CD16/CD32 (clone: 2.4G2)	BD Biosciences	Cat# 553142; RRID:AB_394657
Rat anti-B220 (clone: RA3-6B2) APC-eFluor780	Thermo Fisher Scientific	Cat# 47-0452-82; RRID:AB_1518810
Armenian hamster anti-FAS (clone: Jo2) PE-Cy7	BD Biosciences	Cat# 557653; RRID:AB_396768
Rat anti-mouse T and B cell activation antigen (clone: GL7) FITC	BD Biosciences	Cat# 553666; RRID:AB_394981
Rat anti-CD43 MicroBeads	Miltenyi Biotec	Cat# 130-049-801; RRID:AB_2861373
Rat anti-CD38 (clone: 90) biotin	Thermo Fisher Scientific	Cat# 13-0381-81; RRID:AB_466427
Armenian hamster anti-CD11c (clone: N418) biotin	Thermo Fisher Scientific	Cat# 13-0114-85; RRID:AB_466364
Mouse anti-biotin MicroBeads	Miltenyi Biotec	Cat# 130-090-485; RRID:AB_244365
Biological samples
Citrated sheep red blood cells	Colorado Serum Company	Cat# 31102
Chemicals, peptides, and recombinant proteins
Ammonium chloride (NH4Cl)	Sigma	Cat# A0171
Potassium bicarbonate (KHCO3)	Macron Chemicals	Cat# 6748-03
Ethylenediaminetetraacetic acid (EDTA), disodium salt (C10H18N2Na2O10)	Thermo Fisher Scientific	Cat# S25687
Bovine serum albumin (BSA)	RPI	Cat# A30075
Sodium azide (NaN3)	RPI	Cat# S24080
1× DPBS	Corning	Cat# 21-031-CV
10× DPBS	Corning	Cat# 20-031-CV
Fetal bovine serum (FBS)	Thermo Fisher Scientific	Cat# 26140079
Ethanol (200 proof)	Decon Labs	Cat# 2105
Trypan blue solution 0.4% (w/v)	Corning	Cat# 25-900-CI
7-AAD	BD Biosciences	Cat# 559925
Critical commercial assays
UltraComp eBeads Compensation Beads	Thermo Fisher Scientific	Cat# 01-2222-42
Experimental models: organisms/strains
Mouse: wild-type (C57BL/6)	The Jackson Laboratory	Cat# JAX:000664; RRID:IMSR_JAX:000664
Software and algorithms
FlowJo 10	Becton, Dickinson and Company	https://www.flowjo.com/
FACSDiva	BD Biosciences	https://www.bdbiosciences.com/
Spectrum Viewer	BD Biosciences	https://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer
Other
Pipettes (P2, P20, P200, P1000)	Major Supplier	n/a
Pipette tips (10 μL, 20 μL, 200 μL, 1,000 μL)	Major Supplier	n/a
Pipet controller	Major Supplier	n/a
10-mL serological pipet	Major Supplier	n/a
15-mL conical tube	Sarstedt	Cat# 62.554.205
50-mL conical tube	Sarstedt	Cat# 62.547.205
1.5 mL microcentrifuge tubes	Major Supplier	n/a
Micro dissecting scissors	Roboz	Cat# RS-5990
Micro dissecting forceps	Roboz	Cat# RS-5137
Frosted microscope slides	Thermo Fisher Scientific	Cat# 12-550-343
70 μm Nylon mesh or cell strainer	Major Supplier	n/a
60-mm cell culture dish	Sarstedt	Cat# 83.3901.500
5-mL round bottom tube	Corning	Cat# 352008
1.1-mL mini tubes	Neptune Scientific	Cat# 89092-226
0.22 μm filter	MilliporeSigma	Cat# S2GPU05RE
Insulin syringe	Becton, Dickinson and Company	Cat# 329420
1-mL syringe	Becton, Dickinson and Company	Cat# 309659
18-gauge needle	Becton, Dickinson and Company	Cat# 305195
Hemocytometer	Hausser Scientific	Cat# 3200
LS columns	Miltenyi Biotec	Cat# 130-042-401
QuadroMACS separator (magnet)	Miltenyi Biotec	Cat# 130-090-976
Vortex mixer	Scientific Industries	n/a
Allegra X-15R (refrigerated swing bucket centrifuge)	Beckman Coulter	n/a
Laminar flow hood	Major Supplier	n/a
Magnetic stirrer w/ magnetic stir bar	Major Supplier	n/a
pH meter	Major Supplier	n/a
Scale	Major Supplier	n/a
FACSCanto (flow cytometer)	BD Biosciences	n/a