Mouse embryonic feeders (MEFs) medium (filter sterilized and store at 4°C for up to 1 month)

Reagent	Final concentration	Volume
IMDM	n/a	442.5 mL
MTG (11.56 M)	1.4 × 10−4 M	0.063 mL of a 1/100 dilution
L-Glutamine 100× (200 mM)	2 mM	5 mL
FBS (heat inactivated at 56°C for 30 min)	10%	50 mL
Penicillin (10,000 units), streptomycin (10 mg/mL)	50 units/mL, 0.05 mg/mL	2.5 mL
Total	n/a	500 mL