OP9-DLL1 medium (Filter sterilized and store at 4°C for up to 1 month)

Reagent	Final concentration	Volume
FBS (heat inactivated at 56°C for 30 min)	20%	20 mL
L-Glutamine 200 mM	2 mM	1 mL
α-MEM (pen/strep 1%)	n/a	79 mL
Total	n/a	100 mL