Figure 3.
a) Results of S1‐PFGE for CP66‐6 and its subclones after serial passages in colistin and colistin‐free medium. The lanes of mcr‐positive clones are labeled. C0 denotes clones grown in medium without colistin, while C4 is used for clones grown with 2 mg L−1 colistin. The numbers 7, 14, 21, and 28 indicate the passage number (one passage per day) at which the clone is isolated. The last number indicates the clone number in the plates screened for mcr genes. b) Sequence alignment between the mcr‐1‐bearing chromosomal region of CP66‐6 and the corresponding chromosomic region of the subclone CP66‐6‐C0_7‐3.