Table 1.
Genomic features of the five E. coli strains positive for the mcr‐1 and mcr‐3 genes
| Strain | Contigs a) | ST | Serotype | Resistance genes | Genetic context of mcr genes | Virulence genes | Size (bp) | Plasmid types |
|---|---|---|---|---|---|---|---|---|
| CP8‐3 | CP8‐3‐chromosome | ST34 | – | mcr‐1.1, mdf(A) | ISApl1‐mcr‐1‐Dpap2‐IS1294‐ISApl1; ISKpn19‐TnAs2‐mcr‐3.5‐dgkA‐IS15 | astA, gad | 4.7m | chromosome |
| pCP8‐3‐IncFII | aadA1, aac(3)‐VIa, qnrS1, floR, tet(M) | 87k | IncFII | |||||
| pCP8‐3‐IncFIB | aadA1, aadA2, mef(B), cmlA1, sul3, tet(A), dfrA12 | 75k | IncFIA(HI1), IncFIB(K), IncN | |||||
| pCP8‐3‐IncR | bla TEM‐1B, mcr‐3.5, qnrS1, tet(M) | 47k | IncR | |||||
| pCP8‐3‐IncX1 | – | 38k | IncX1 | |||||
| pCP8‐3‐IncQ | sul2 | 8k | IncQ1 | |||||
| CP55 | CP55‐chromosome | ST971 | O128:H27 | aac(3)‐IId, qnrS1, mdf(A), floR, catA1 | mcr‐1‐pap2; IS4321R‐TnAs2‐mcr‐3.5‐dgkA‐IS15 | capU, cma, gad, iha, ireA, iroN, iss, lpfA, mchF, subA | 4.9m | chromosome |
| pCP55‐IncFIB | aph(3″)‐Ib, aph(6)‐Id, bla TEM‐1B, sul2, tet(A) | 156k | IncFIB, IncFII | |||||
| pCP55‐141k | – | 141k | IncB/O/K/Z | |||||
| pCP55‐IncFII | mcr‐3.5, mph(A) | 70k | IncFII | |||||
| pCP55‐IncX4 | mcr‐1.1 | 33k | IncX4 | |||||
| CP61 | CP61‐chromosome | – | – | mcr‐1.1, mdf(A) | Tn6330; TnAs2‐mcr‐3.1‐dgkA‐ISKpn40 | gad | 4.5m | chromosome |
| pCP61‐IncN | aph(3′)‐Ia, qnrS2, tet(A) | 80k | ColE10, IncFIA(HI1), IncN,IncR | |||||
| pCP61‐IncFIB | aadA2, aadA1, aac(3)‐VIa, bla TEM‐1B, mcr‐3.1, mef(B), cmlA1, floR, sul3, tet(M), dfrA12 | 92k | IncFIB(K), IncN | |||||
| CP66‐6 | CP66‐6‐chromosome | ST5229 | – | mcr‐1.1, mdf(A) | ISApl1‐mcr‐1‐Dpap2‐IS1294‐ISApl1; bla TEM‐1B‐TnAs2‐mcr‐3.5‐dgkA‐IS26(IS15DI) | astA, gad, lpfA | 4.8m | chromosome |
| pCP66‐6‐IncFIC | aadA2, aph(3′)‐Ia, aadA17, aac(3)‐IId, aadA1, blaTEM‐215, lnu(F), cmlA1, floR, sul2, sul3, tet(A), tet(M), dfrA12 | 99k | IncFIB(AP001918), IncFIC(FII) | |||||
| pCP66‐6‐IncFII | bla TEM‐215, mcr‐3.5, fosA4, mph(A), dfrA12 | 74k | ColE10, IncFII | |||||
| pCP66‐6‐IncX3 | bla OXA‐181, qnrS1 | 50k | ColKP3, IncX3 | |||||
| pCP66‐6‐IncQ | sul2 | 8k | IncQ1 | |||||
| CP131 | CP131‐chromosome | ST48 | – | mcr‐1.1, mdf(A) | Tn6330 (ISApl1‐mcr‐1‐pap2‐ISApl1); TnAs2‐mcr‐3.1‐dgkA‐ISKpn40 | gad, lpfA | 4.5m | chromosome |
| pCP131‐IncHI1 | aph(3″)‐Ib, aac(3)‐IId, bla TEM‐1B, mcr‐3.1, qnrS1, mef(B), floR, sul3, tet(M) | 264k | IncFIA(HI1), IncHI1A, IncHI1B(R27) | |||||
| pCP131‐IncFIB | aadA2, bla TEM‐1B, tet(A), mph(A), floR, sul3, tet(M), dfrA12 | 116k | IncFIB(AP001918), IncFIC(FII) |
a)
All contigs were circular plasmids and chromosomes. Plasmids smaller than 10 kb without resistance genes are not listed here. For the complete sequencing data, refer to the figshare database (https://doi.org/10.6084/m9.figshare.11825871). The dash symbols denote unidentified ST, serotypes, and resistance genes.