FIGURE 7.

BMP9 induces ectopic bone formation from iTGMCs in vivo. A, BMP9 induced ectopic bone formation from iTGMCs in vivo was analysed by μCT. No masses were detectable in the Ad‐GFP‐infected iTGMC ‐KD and iTGMC‐Ctrl groups (a). The average bone volume was determined using the vivaCT 40, uCT V6.1 software(b). (*P < .05). B, Haematoxylin and eosin (H & E) staining showed that when transduced with BMP9 or in the presence of both BMP9 and PPCNg, trabecular bone formation was remarkably repressed in iTGMC‐KD compared with iTGMC‐Ctrl. C, Trichrome staining revealed that mature and mineralized bone matrices formation were also remarkably repressed in iTGMC‐KD cells compared with iTGMC‐Ctrl. MBM, mineralized bone matrix; TB, trabecular bone; OM, osteoid matrix. Bar = 50µM