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. 2021 Feb 15;13(3):e12005. doi: 10.15252/emmm.202012005

Figure EV3. Effect of the combination of COCO and VEGF inhibition on angiogenesis.

Figure EV3

  1. Representative images of HUVECs sprouting in a fibrin gel with or without Flt1Fc (100 ng/ml) in the presence or absence of COCO. Scale bar, 75 μm.
  2. Quantification of the number of junctions/field of images shown in (A). *P = 0.0378 (Untreated vs. Flt1Fc); **P = 0.0069 (COCO50 ng/ml vs. COCO50 ng/ml + Flt1Fc); (n = 3; 5 pictures/experiment).
  3. Retinal flat mounts of P5 mice injected with PBS, COCO, or Flt1Fc alone or in combination are stained with IB4 (negative images of the fluorescent signal). Scale bar, 100 μm.
  4. Quantification of number of branchpoints. Results are presented as mean ± SEM and statistical significance was analyzed by two‐way ANOVA. *P = 0.0151 (PBS vs. Flt1Fc); **P = 0.0060 (PBS vs. COCO); **P = 0.0079 (PBS vs. COCO + Flt1Fc); n.s. = non significant; (n = 7).