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. 2021 Feb 17;13(3):e13549. doi: 10.15252/emmm.202013549

Figure 2. Flow cytometry of Jurkat‐S cells allows to detect anti‐S immunoglobulins poorly detected by commercial tests with a wide dynamic range.

Figure 2

  • A
    Overlay histograms of Jurkat‐S staining with different human sera diluted 1:50. A pre‐COVID‐19 serum sample is taken as negative control (gray histogram).
  • B
    Bar plots of flow cytometry data generated with Jurkat‐S cells and the panel of serum samples of Table EV1 classified according to their result in the indicated commercial tests: green, positive for the test; orange, weak, or unclear; magenta, negative samples. Flow cytometry data are expressed as the ratio between the MFI of the antibody anti‐S and the MFI for EGFR. Negative values for the flow cytometry test are those with a S/EGFR MFI ratio lower than 0.5. This ratio was set in order to fit most of the data negative for the other serological tests (pink triangles) under that threshold. Data represent the mean ± s.e.m. All datapoints are shown.