Figure EV2. Flow cytometry of Jurkat‐S cells can be used in multiplexed methods to detect simultaneously the expression of anti‐S antibodies of all subclasses.

1. Detection of anti‐S immunoglobulins of the indicated subclasses in a multiplexed method of flow cytometry in the serum samples indicated at 1:50 dilution. Data represent the mean ± SD of triplicated datasets.
2. Rainbow heatmap plot of the anti‐S protein antibody data shown in (A), normalized as fold value of the S/egfr MFI ratio in each sample divided by the mean S/egfr MFI ratio of the pre‐COVID‐19 serum.