TABLE 3.
The descriptions, ADF test results and values of the morphokinetic parameters
| Structure | Morphokinetic parameters | Description | Selection based | Number of samples followed stationary hypothesis (%) | Stationary or non‐stationary parameter | Value of the parameters (n=24) c |
|---|---|---|---|---|---|---|
| Cytoplasm | cm_size | Size of cytoplasm | (Zhao, et al., 2019) 40 | 0/24(0) | Non‐stationary | 10073.35 ± 689.19 μm 2c |
| cm_r_mean | Mean of cytoplasm radius | Derivation | 0/24(0) | Non‐stationary | 56.37 ± 1.93 μm c | |
| cm_r_std | Deviation of cytoplasm radius | Derivation | 0/24(0) | Non‐stationary | 1.45 (0.99‐2.3) | |
| cm_i_mean | Mean of cytoplasm greyscale | (Scott, 2003) 41 | 0/24(0) | Non‐stationary | 119.37 (108.88‐132.87) | |
| cm_i_std | Deviation of cytoplasm greyscale | Derivation | 0/24(0) | Non‐stationary | 40.69 (37.86‐43.11) | |
| ZP | zp_thickness | 75th and above of ZP thickness | (Rienzi, Vajta, &Ubaldi, 2011) 10 | 24/24(100) | Stationary | 16.92 (15.75‐18.05) μm |
| zp_thickness_std | Deviation of ZP thickness | Derivation | 23/24(95.83) b | Stationary | 3.36 (2.13‐4.43) | |
| zp_g_mean | Mean of ZP greyscale | (Sauerbrun‐Cutler, et al., 2015) 42 | 23/24(95.83) b | Stationary | 137.04 (121.69‐154.37) | |
| zp_g_std | Deviation of ZP greyscale | Derivation | 23/24(95.83) b | Stationary | 30.54 (25.77‐35.26) | |
| pvs_mean | Mean of perivitelline space | (Rienzi, et al., 2008) 43 | 1/24(4.17) b | Non‐stationary | 2.7 (1.96‐3.64) μm | |
| PN | pn1_sz a | Mean value of PN size | (Scott, 2003) 41 | 0/24(0) d | Non‐stationary d | 503.19 (434.38‐557.54) μm 2d |
| pn2_sz a | Mean value of PN size | (Scott, 2003) 41 | ||||
| pn _dist a | Distance of pn1 to pn2 | (Beuchat, et al., 2008) 13 | 0/24(0) | Non‐stationary | 21.3 (18.59‐23.78) μm | |
| pn1_dist_cen a | The distance of pn1 to the geometrical centroid of cytoplasm | Derivation | 0/24(0) | Non‐stationary | 9.25 (7.32‐10.87) μm | |
| pn2_dist_cen a | The distance of pn2 to the geometrical centroid of cytoplasm | Derivation | 0/24(0) | Non‐stationary | 14.24 (12.5‐16.85) μm | |
| pn_fading | The duration from fertilization to PN disappearance | (Kaser and Racowsky, 2014) 44 |
We selected morphokinetic parameters of cytoplasm, ZP and PN. The selections were based on the literature and on our previous studies. Stationary tests were conducted and parameters with the proportion higher than 70% following the hypothesis were considered as non‐stationary parameters. The descriptions and values of the parameters were given in the table as well.
a
The pn1 was defined as the PN that closer to the centroid of the cytoplasm.
b
The portion of accepting the stationary hypothesis is larger than 30%.
c
Normal distribution.
d
pn_size_med.