Figure 3. Selection and depletion of the edited T cells by exploiting an optimized hEGFRt gene.

1. Schematics of donor DNA templates carrying corrective cDNA coupled to IRES sequence and hEGFRt selector genes.
2. Representative plots showing hEGFRt expression in bulk edited CD4⁺ T cells derived from male HD before and 8 h after PMA/Ionomycin stimulation. Cells were edited with the three constructs depicted in (A).
3. Representative plots showing EGFR expression in bulk edited CD4⁺ T cells derived from male HD at 3 days after treatment with immunotoxin (left), antibody (middle), or toxin (right).
4. Bar plot showing percentage of EGFR⁺ T cells at 3 days after treatment with 5 nM or 1 nM of immunotoxin, antibody, or toxin, measured by FACS Analysis. Friedman test with Dunn’s multiple comparisons. P‐values were adjusted with Bonferroni’s correction to account for multiple comparisons (**P = 0.0052 and **P = 0.0024 for immunotoxin vs. antibody and **P = 0.0010 and **P = 0.0024 for immunotoxin vs. toxin, referring to EGFRmod1 and EGFRmod2, respectively). Different dose conditions were used as a unified group for statistical analysis (n = 10 for each group). Median ± IQR.

Source data are available online for this figure.