Fig. 5. DDX5 is a target of miR-206. (A) Venn diagram depicts the number of candidate target genes of miR-206. (B) Expression of candidate mRNAs in LPS-induced HK-2 cells was detected by RT-qPCR. (C) Binding sequences were predicted by TargetScan, and the combination between miR-206 and DDX5 3'UTR was confirmed by luciferase reporter assay. (D) Western blot analysis was conducted to measure DDX5 protein levels in the condition of miR-206 overexpression. (E) RT-qPCR to detect the mRNA levels of DDX5 in sera from septic patients with AKI (compared to healthy individuals). (F) Western blot analysis was conducted to detect protein expression of DDX5 in kidney tissues in CLP mice (compared to sham mice). *p<0.05. DDX5, DEAD-box helicase 5; RT-qPCR, reverse transcription quantitative polymerase chain reaction; LPS, lipopolysaccharide; UTR, untranslated region; AKI, acute kidney injury; CLP, cecal ligation and puncture.
