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. 2021 Feb 23;45(4):40. doi: 10.3892/or.2021.7991

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Rescue assay. (A) GH3 cells were divided into 3 groups (shNC + miR-NC, shMEG3 + miR-NC and shMEG3 + miR-23b-3p inhibitor). Cell Counting Kit-8 assay was performed to assess proliferation. (B) Apoptosis was verified by flow cytometry assay. (C) Transwell assay was performed to detect cell migration and invasion (magnification, ×200). (D) Western blot analysis was performed to assess the levels of apoptosis- and EMT-associated proteins. **P<0.01. sh, short hairpin; NC, negative control; miR, microRNA; OD, optical density.