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. 2021 Feb 26;45(4):45. doi: 10.3892/or.2021.7996

Figure 1.

Figure 1.

Circ_0000003 is upregulated in TSCC tissues and cell lines and promotes TSCC cell proliferation in vitro. (A) Circ_0000003 level was examined in TSCC tissues (n=40) and their paired adjacent nontumor tissues (n=40). **P<0.01 vs. the nontumor tissues. (B) Circ_0000003 level was measured in HOK and TSCC cells (SCC25, SCC4, Cal27 and SCC1). **P<0.01 vs. the HOK. (C) Survival rates of TSCC patients with high and low circ_0000003 expression were analyzed via Kaplan-Meier survival analysis. (D) Circ_0000003 shRNA (sh-circ_0000003) transfection silenced the circ_0000003 expression in Cal27 and SCC1 cells. (E) CCK-8 assay was conducted to detect the cell proliferative ability of Cal27 and SCC1 cells with circ_0000003 knockdown. (F) p-circ_0000003 transfection enforced the circ_0000003 expression in SCC25 and SCC4 cells. (G) CCK-8 assay was conducted to detect the cell proliferative ability of SCC25 and SCC4 cells with circ_0000003 overexpression. *P<0.05 and **P<0.01 vs. controls. circ_0000003, hsa_circ_0000003; TSCC, tongue squamous cell carcinoma; HOK, human oral keratinocytes; sh-circ_0000003, circ_0000003 shRNA; CCK-8, Cell Counting Kit-8; p-circ_0000003, pcDNA3.1-circ_0000003; sh, shRNA; NC, negative control.