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. 2021 Feb 26;45(4):45. doi: 10.3892/or.2021.7996

Figure 4.

Figure 4.

Circ_0000003 regulates miR-330-3p and GLS expressions, and miR-330-3p regulates GLS expression. (A and B) RT-qPCR determined the miR-330-3p expression in Cal27 and SCC1 cells transfected with sh-NC or sh-circ_0000003, and in SCC25 and SCC4 cells transfected with empty vector or p-circ_0000003. (C and D) RT-qPCR determined the miR-330-3p and GLS mRNA expression in Cal27 and SCC1 cells transfected with control mimic or miR-330-3p mimic. (E and F) RT-qPCR determined the miR-330-3p and GLS mRNA expression in SCC25 and SCC4 cells transfected with miRNA inhibitor-NC or miR-330-3p inhibitor. (G) Western blot analysis determined the GLS protein expression in Cal27 and SCC1 cells transfected with control mimic or miR-330-3p mimic. Lane 1, blank control; lane 2, control mimic; lane 3, miR-330-3p mimic. (H) Western blot analysis determined the GLS protein expression in SCC25 and SCC4 cells transfected with miRNA inhibitor-NC or miR-330-3p inhibitor. Lane 1, blank control; lane 2, miRNA inhibitor-NC; lane 3, miR-330-3p inhibitor. (I and J) RT-qPCR determined the GLS mRNA expression in Cal27 and SCC1 cells transfected with sh-NC or sh-circ_0000003, and in SCC25 and SCC4 cells transfected with empty vector or p-circ_0000003. (K) Western blot analysis determined the GLS protein expression in Cal27 and SCC1 cells transfected with sh-NC or sh-circ_0000003. Lane 1, blank control; Lane 2, sh-NC; Lane 3, sh-circ_0000003. (L) Western blot analysis determined the GLS protein expression in SCC25 and SCC4 cells transfected with empty vector or p-circ_0000003. Lane 1, blank control; lane 2, vector control; lane 3, p-circ_0000003. **P<0.01 vs. controls. circ_0000003, hsa_circ_0000003; GLS, glutaminase; RT-qPCR, reverse transcription and quantitative polymerase chain reaction; sh, shRNA; miRNA, microRNA; NC, negative control; sh-circ_0000003, circ_0000003 shRNA; p-circ_0000003, pcDNA3.1-circ_0000003.