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. 2021 Feb 25;45(4):44. doi: 10.3892/or.2021.7995

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Cx43 mRNA and protein levels in U251s and U251r cells. (A and B) Cx43 mRNA levels in U251s and U251r cells were detected by reverse transcription-quantitative PCR. The transcription of GAPDH was used as endogenous control. (C and D) Cx43 protein expression level in U251s and U251r cells. GAPDH was used as endogenous control. (B and D) Cx43 mRNA and protein levels in U251s and U251r cells were quantitatively analyzed from data of at least three independent experiments. **P<0.01. Cx43, connexin 43; U251r, temozolomide-resistant U251 glioma cells; U251s, temozolomide-sensitive U251 glioma cells; RFU, relative fluorescence units.