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. 2021 Feb 25;45(4):44. doi: 10.3892/or.2021.7995

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of exosomal Cx43 on TMZ resistance and colony formation ability of U251s cells. (A) U251s cell viability in each group was detected using Cell Counting Kit-8 assay at 72 h after treatment with TMZ, and the cell viability at each TMZ concentration was plotted for the dose-response curve. (B) IC₅₀ values of U251s cells to TMZ in the PBS, sExo, rExo and rExo + ^37,43Gap27 groups. Data represent the mean of at least three independent experiments for each condition. **P<0.01, ***P<0.001. (C) Colony formation assay was conducted in U251s cells treated with PBS, sExo, rExo and rExo + ^37,43Gap27. The representative images showed the colony morphology in each group. (D) Quantification of the number of colonies for statistical analysis. Data represent the results of three independent experiments for each group. **P<0.01, ***P<0.001. Cx43, connexin 43; TMZ, temozolomide; U251r, TMZ-resistant U251 glioma cells; U251s, TMZ-sensitive U251 glioma cells; sExo, exosomes of U251s cells; rExo, exosomes of U251r cells.