Skip to main content
. 2021 Feb 25;45(4):44. doi: 10.3892/or.2021.7995

Figure 6.

Figure 6.

Exosomal Cx43 enhances the migration ability of U251s cells. (A) Wound healing experiments were performed to evaluate the migration of U251s cells at 0, 24 and 48 h after treatment with PBS, sExo, rExo and rExo + 37,43Gap27. Scale bar, 200 µm. (B) Wound closure rate was measured using ImageJ software for statistical analysis. Data represent the results of at least three independent experiments for each group. **P<0.01, ***P<0.001. (C) Transwell assay was used to examine the migration of U251s cells after treatment with PBS, sExo, rExo and rExo + 37,43Gap27. Scale bar, 200 µm. (D) The migrated U251s cells were counted in at least 9 fields from three independent experiments for each treatment. **P<0.01. Cx43, connexin 43; U251r, temozolomide-resistant U251 glioma cells; U251s, temozolomide-sensitive U251 glioma cells; sExo, exosomes of U251s cells; rExo, exosomes of U251r cells.