Fig. 4.

CircCSPP1 contributes to cell progression and DOX-resistant by sponging miR-944. a–j LoVo/DOX and HCT116/DOX cells were transfected with si-NC, si-circCSPP1, si-circCSPP1 + in-miR-NC, or si-circCSPP1 + in-miR-944, respectively. a MiR-944 expression was detected by qRT-PCR in transfected LoVo/DOX and HCT116/DOX cells. b The transfected LoVo/DOX and HCT116/DOX cells were analyzed by MTT assay. IC50 of 24 h was shown. c–e Cell proliferation was assessed by MTT assay c, d and colony formation assay (e). f, g Transwell assay was performed to analyze cell migration (f) and invasion (g). h Flow cytometry analysis was used to examine cell apoptosis. i, j The protein levels of MRP1, P-gp and LRP were measured by western blot assay. *P < 0.05