Fig. 5.

MiR-944 directly target to FZD7. a DIANA tool was used to predict potential target genes of miR-944. The red trace is the predicted binding sites and the blue mark represents the mutant sites in FZD7 3′-UTR. b, c Relative luciferase activity was determined in both LoVo/DOX and HCT116/DOX cells co-transfected with miR-944 or miR-NC and FZD7 3′UTR WT or FZD7 3′UTR MUT. d, e The mRNA and protein level of FZD7 was detected by qRT-PCR assay in DOX-resistant and DOX-sensitive CRC tissues (d) or DOX-resistant and normal CRC cells (e). f, g Western blot assay was performed to measure the protein level of FZD7 in cells transfected with miR-944 (f) or si-circCSPP1 (g). *P < 0.05