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. 2021 Mar 4;20:60. doi: 10.1186/s12934-021-01550-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Sequence alignment among previously characterized ketose 3-epimerases. The amino acid source and GenBank accession number are as follows: Rhodopirellula baltica (WP_007330622.1); Arthrobacter globiformis (BAW27657.1); Staphylococcus aureus (SQA09501.1); Rhodobacter sphaeroides (ACO59490.1); Sinorhizobium sp. (WP_069063284.1); Pseudomonas cichorii (BAA24429.1); Caballeronia fortuita (WP_061137998.1); Flavonifractor plautii (EHM40452.1); DaeM (MN337631); Bacillus sp. (DaeB) (KYG89858.1); Desmospora sp. 8437 (WP_009711885.1); Paenibacillus senegalensis (WP_010270828.1); Clostridium bolteae (EDP19602.1); Treponema primitia ZAS-1 (ZP_09717154.1); Ruminococcus sp. (ZP_04858451.1); Agrobacterium tumefaciens (AAK88700.1); Agrobacterium sp. ATCC 31749 (EGL65884.1); Clostridium cellulolyticum H10 (ACL75304.1); Clostridium scindens (WP_004607502.1); Dorea sp. CAG317 (WP_022318236.1). The conserved amino acid residues critical in d-fructose epimerization are depicted in green color box