Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 4;23:29. doi: 10.1186/s13058-021-01406-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — ALRN-6924 combined with paclitaxel reactivates p53 and induces cell cycle arrest and apoptosis in vitro. a Cell cycle analysis was carried out by flow cytometry after 24-h treatment with vehicle, ALRN-6924 (10 μM), paclitaxel (1 μM), or combination of both. Percentages of cells in Sub-G1, G0-G1, S, and G2-M phases of cell cycle are depicted. b Flow cytometry analysis of Annexin V-positive cells after treatment for 72 h by vehicle, ALRN-6924 (10 μM), paclitaxel (1 μM), or combination of both in both MCF-7 and ZR-75-1. Mean ± SD (n = 3) values are shown. c Immunoblotting of p53, p21, Mdm2, and p-Histone-H3 (Ser10). MCF-7 and ZR-75 were treated with vehicle control, ALRN-6924 at 10 μM, or paclitaxel 0.1, 1 μM, or combination of both for 24 h