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. 2021 Mar 4;9:17. doi: 10.1186/s40364-021-00270-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Downregulation of PTK7 induces cell growth retardation and apoptosis. (a) The BCL11B and PTK7 mRNA expression levels in T-ALL (Jurkat and Molt-4) and non-T-ALL (Raji) cell lines. GAPDH was used as an endogenous reference. (b) Cultured Jurkat and Raji cells were treated with Cy5-Sgc8 for 1 h at different concentrations. Flow cytometry analysis of the specific cell binding of Cy5-Sgc8 to Jurkat cells. (c) Expression of PTK7 in T-ALL cell lines 48 h after BCL11B siRNA transfection. (d) Effect of BCL11B siRNA on the BCL11B mRNA expression level by real-time quantitative polymerase chain reaction (qRT-PCR) 48 h after transfection in CB CD3+ T cells. Negative control siRNA-treated cells were used for comparison. (e) Knockdown efficiency in Molt-4 cells were analyzed after knocking down the PTK7 gene. (f) Molt-4 cells were transfected with si-PTK7 and then cell proliferation was assessed by Cell counting kit-8 (CCK-8) assay. (g) Apoptosis in Molt-4 cells transfected with si-PTK7 was measured by flow cytometry after 48 h. (h) Expression of the TRAIL and p27 genes in Molt-4 cells 48 h after transfected with BCL11B siRNA and PTK7 siRNA. Non-specific siRNA treated cells were used as negative control. Asterisks signify statistically significant differences (***, P < 0.001; **, P < 0.01; *, P < 0.05)