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. 2021 Mar 5;40:89. doi: 10.1186/s13046-021-01883-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — P53-dependent SASP induction after irradiation. a P53 and p21 expression in A549, A549sh/p53 and H1299 cells after different radiation doses detected by western blot. The image is representative at least of 2 independent experiments. b Representative images of senescence-associated β-galactosidase (SA-β-gal) staining detected after A549, A549sh/p53, and H1299 cell exposure to 10 or 20 Gy. Scale bar is 100 μm. c mRNA expression of SASP biomarkers using GAPDH and HPRT as housekeeping genes. Data are presented as mean ± SD. d EV quantification using Nanosight nanoparticle tracking analysis. EVs were isolated from the conditioned media of A549, A549sh/p53 and H1299 cells, as described in the Methods section (*p < 0.05)